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A microtome was used to cut the embedded samples into 60 nm ultrathin sections, which were then stained with 2% uranyl acetate at room temperature in the dark for 20 min and lead citrate at room temperature for 10 min
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Growth hormone production begins declining around age 30, with roughly 15% reduction per decade thereafter
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Hogg E, Wu T, Bresee C, Wertheimer J, Malatt C, Tan E, et al
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